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macsquant analyzer 10 flow cytometer  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec macsquant analyzer 10 flow cytometer
    Macsquant Analyzer 10 Flow Cytometer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 98/100, based on 2038 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flow+cytometer/MACSQuant+Analyzer+10/pm42448954-283-24-30
    Average 98 stars, based on 2038 article reviews
    macsquant analyzer 10 flow cytometer - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Incubation:

    Article Title: Impact of rifampicin on P-glycoprotein (ABCB1) expression in M1 and M2 macrophages derived from the THP-1 monocytic cell line or peripheral blood mononuclear cells
    Article Snippet: The obtained pellet was resuspended again in DPBS and the cell count of monocytes contained therein was determined using a flow cytometer (MACSQuant analyzer 10, Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Tunnelling Nanotube-Mediated Lysosome Sharing Promotes Osteocyte Survival via Transcellular Autophagy.
    Article Snippet: Samples were acquired using a flow cytometer (MACSQuant Analyzer 16, Miltenyi Biotec, USA) or sorted by fluorescence- activated cell sorting (FACS) using a BD Influx (BD Biosciences, USA).

    Article Title: Rifabutin boosts rifampicin accumulation in THP-1-derived M2 macrophages by inhibiting P-glycoprotein efflux activity.
    Article Snippet: Following this efflux phase, cells were washed two times and median fluorescence was recorded using a flow cytometer (MACSQuant analyzer 10, Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Clinical validation of CD16b as a standardized biomarker for inherited GPI deficiencies.
    Article Snippet: The stained cells were then analyzed by a flow cytometer (MACSQuant VYB, Miltenyi Biotec) to detect GPI-APs on granulocytes.

    Article Title: Influence of stabilizer, size and physical state of lipid nanoparticles on uptake by THP-1 macrophages.
    Article Snippet: The samples were protected from light and stored on ice until they were analyzed with a flow cytometer (MACS Quant VYB, Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Response of the bovine oviduct epithelium to sperm binding and proximity: a region-specific proteomic approach using spheroids.
    Article Snippet: Spermatozoa were then analyzed using a flow cytometer (Miltenyi Biotec MACSQuant Analyzer 10).

    Article Title: Development of highly selective, low-toxicity and orally available Mcl-1 inhibitors based on 3,5-dimethyl-4-sulfonimide-1H-pyrrole scaffold via P2/P3 dual pocket binding optimization.
    Article Snippet: Myeloid cell leukemia 1 (Mcl-1) is a pivotal anti-apoptotic protein, whose overexpression drives tumorigenesis, progression and drug resistance in multiple hematological malignancies.. Herein, guided by a bioisosteric design, we replaced the sulfonyl group with a drug-like sulfonimide linker and synthesized a novel series of Mcl-1 inhibitors based on our previous lead compound DDO-8201.. Among them, compound 52 displayed potent and selective Mcl-1 inhibition (Ki = 0.024 μM) with at least 400-fold selectivity over other Bcl-2 family proteins.

    Article Title: Metabolic rewiring driven by phosphoglycolate phosphatase deletion inhibits ferroptosis
    Article Snippet: Cell viability was assessed after 16 hours using resazurin (Cayman Chemical) or AquaBluer (MoBiTec), according to the manufacturer’s recommendations.

    Flow Cytometry:

    Article Title: Impact of rifampicin on P-glycoprotein (ABCB1) expression in M1 and M2 macrophages derived from the THP-1 monocytic cell line or peripheral blood mononuclear cells
    Article Snippet: The obtained pellet was resuspended again in DPBS and the cell count of monocytes contained therein was determined using a flow cytometer (MACSQuant analyzer 10, Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Tunnelling Nanotube-Mediated Lysosome Sharing Promotes Osteocyte Survival via Transcellular Autophagy.
    Article Snippet: Samples were acquired using a flow cytometer (MACSQuant Analyzer 16, Miltenyi Biotec, USA) or sorted by fluorescence- activated cell sorting (FACS) using a BD Influx (BD Biosciences, USA).

    Article Title: Rifabutin boosts rifampicin accumulation in THP-1-derived M2 macrophages by inhibiting P-glycoprotein efflux activity.
    Article Snippet: Following this efflux phase, cells were washed two times and median fluorescence was recorded using a flow cytometer (MACSQuant analyzer 10, Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Clinical validation of CD16b as a standardized biomarker for inherited GPI deficiencies.
    Article Snippet: The stained cells were then analyzed by a flow cytometer (MACSQuant VYB, Miltenyi Biotec) to detect GPI-APs on granulocytes.

    Article Title: Influence of stabilizer, size and physical state of lipid nanoparticles on uptake by THP-1 macrophages.
    Article Snippet: The samples were protected from light and stored on ice until they were analyzed with a flow cytometer (MACS Quant VYB, Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Response of the bovine oviduct epithelium to sperm binding and proximity: a region-specific proteomic approach using spheroids.
    Article Snippet: Spermatozoa were then analyzed using a flow cytometer (Miltenyi Biotec MACSQuant Analyzer 10).

    Article Title: Development of highly selective, low-toxicity and orally available Mcl-1 inhibitors based on 3,5-dimethyl-4-sulfonimide-1H-pyrrole scaffold via P2/P3 dual pocket binding optimization.
    Article Snippet: Myeloid cell leukemia 1 (Mcl-1) is a pivotal anti-apoptotic protein, whose overexpression drives tumorigenesis, progression and drug resistance in multiple hematological malignancies.. Herein, guided by a bioisosteric design, we replaced the sulfonyl group with a drug-like sulfonimide linker and synthesized a novel series of Mcl-1 inhibitors based on our previous lead compound DDO-8201.. Among them, compound 52 displayed potent and selective Mcl-1 inhibition (Ki = 0.024 μM) with at least 400-fold selectivity over other Bcl-2 family proteins.

    Article Title: Metabolic rewiring driven by phosphoglycolate phosphatase deletion inhibits ferroptosis
    Article Snippet: Cell viability was assessed after 16 hours using resazurin (Cayman Chemical) or AquaBluer (MoBiTec), according to the manufacturer’s recommendations.

    Fluorescence:

    Article Title: Impact of rifampicin on P-glycoprotein (ABCB1) expression in M1 and M2 macrophages derived from the THP-1 monocytic cell line or peripheral blood mononuclear cells
    Article Snippet: The obtained pellet was resuspended again in DPBS and the cell count of monocytes contained therein was determined using a flow cytometer (MACSQuant analyzer 10, Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Tunnelling Nanotube-Mediated Lysosome Sharing Promotes Osteocyte Survival via Transcellular Autophagy.
    Article Snippet: Samples were acquired using a flow cytometer (MACSQuant Analyzer 16, Miltenyi Biotec, USA) or sorted by fluorescence- activated cell sorting (FACS) using a BD Influx (BD Biosciences, USA).

    Article Title: Rifabutin boosts rifampicin accumulation in THP-1-derived M2 macrophages by inhibiting P-glycoprotein efflux activity.
    Article Snippet: Following this efflux phase, cells were washed two times and median fluorescence was recorded using a flow cytometer (MACSQuant analyzer 10, Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Clinical validation of CD16b as a standardized biomarker for inherited GPI deficiencies.
    Article Snippet: The stained cells were then analyzed by a flow cytometer (MACSQuant VYB, Miltenyi Biotec) to detect GPI-APs on granulocytes.

    Article Title: Influence of stabilizer, size and physical state of lipid nanoparticles on uptake by THP-1 macrophages.
    Article Snippet: The samples were protected from light and stored on ice until they were analyzed with a flow cytometer (MACS Quant VYB, Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Response of the bovine oviduct epithelium to sperm binding and proximity: a region-specific proteomic approach using spheroids.
    Article Snippet: Spermatozoa were then analyzed using a flow cytometer (Miltenyi Biotec MACSQuant Analyzer 10).

    Article Title: Development of highly selective, low-toxicity and orally available Mcl-1 inhibitors based on 3,5-dimethyl-4-sulfonimide-1H-pyrrole scaffold via P2/P3 dual pocket binding optimization.
    Article Snippet: Myeloid cell leukemia 1 (Mcl-1) is a pivotal anti-apoptotic protein, whose overexpression drives tumorigenesis, progression and drug resistance in multiple hematological malignancies.. Herein, guided by a bioisosteric design, we replaced the sulfonyl group with a drug-like sulfonimide linker and synthesized a novel series of Mcl-1 inhibitors based on our previous lead compound DDO-8201.. Among them, compound 52 displayed potent and selective Mcl-1 inhibition (Ki = 0.024 μM) with at least 400-fold selectivity over other Bcl-2 family proteins.

    Article Title: Metabolic rewiring driven by phosphoglycolate phosphatase deletion inhibits ferroptosis
    Article Snippet: Cell viability was assessed after 16 hours using resazurin (Cayman Chemical) or AquaBluer (MoBiTec), according to the manufacturer’s recommendations.

    FACS:

    Article Title: Impact of rifampicin on P-glycoprotein (ABCB1) expression in M1 and M2 macrophages derived from the THP-1 monocytic cell line or peripheral blood mononuclear cells
    Article Snippet: The obtained pellet was resuspended again in DPBS and the cell count of monocytes contained therein was determined using a flow cytometer (MACSQuant analyzer 10, Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Tunnelling Nanotube-Mediated Lysosome Sharing Promotes Osteocyte Survival via Transcellular Autophagy.
    Article Snippet: Samples were acquired using a flow cytometer (MACSQuant Analyzer 16, Miltenyi Biotec, USA) or sorted by fluorescence- activated cell sorting (FACS) using a BD Influx (BD Biosciences, USA).

    Article Title: Rifabutin boosts rifampicin accumulation in THP-1-derived M2 macrophages by inhibiting P-glycoprotein efflux activity.
    Article Snippet: Following this efflux phase, cells were washed two times and median fluorescence was recorded using a flow cytometer (MACSQuant analyzer 10, Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Clinical validation of CD16b as a standardized biomarker for inherited GPI deficiencies.
    Article Snippet: The stained cells were then analyzed by a flow cytometer (MACSQuant VYB, Miltenyi Biotec) to detect GPI-APs on granulocytes.

    Article Title: Influence of stabilizer, size and physical state of lipid nanoparticles on uptake by THP-1 macrophages.
    Article Snippet: The samples were protected from light and stored on ice until they were analyzed with a flow cytometer (MACS Quant VYB, Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Response of the bovine oviduct epithelium to sperm binding and proximity: a region-specific proteomic approach using spheroids.
    Article Snippet: Spermatozoa were then analyzed using a flow cytometer (Miltenyi Biotec MACSQuant Analyzer 10).

    Article Title: Development of highly selective, low-toxicity and orally available Mcl-1 inhibitors based on 3,5-dimethyl-4-sulfonimide-1H-pyrrole scaffold via P2/P3 dual pocket binding optimization.
    Article Snippet: Myeloid cell leukemia 1 (Mcl-1) is a pivotal anti-apoptotic protein, whose overexpression drives tumorigenesis, progression and drug resistance in multiple hematological malignancies.. Herein, guided by a bioisosteric design, we replaced the sulfonyl group with a drug-like sulfonimide linker and synthesized a novel series of Mcl-1 inhibitors based on our previous lead compound DDO-8201.. Among them, compound 52 displayed potent and selective Mcl-1 inhibition (Ki = 0.024 μM) with at least 400-fold selectivity over other Bcl-2 family proteins.

    Article Title: Metabolic rewiring driven by phosphoglycolate phosphatase deletion inhibits ferroptosis
    Article Snippet: Cell viability was assessed after 16 hours using resazurin (Cayman Chemical) or AquaBluer (MoBiTec), according to the manufacturer’s recommendations.

    Magnetic Cell Separation:

    Article Title: Impact of rifampicin on P-glycoprotein (ABCB1) expression in M1 and M2 macrophages derived from the THP-1 monocytic cell line or peripheral blood mononuclear cells
    Article Snippet: The obtained pellet was resuspended again in DPBS and the cell count of monocytes contained therein was determined using a flow cytometer (MACSQuant analyzer 10, Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Tunnelling Nanotube-Mediated Lysosome Sharing Promotes Osteocyte Survival via Transcellular Autophagy.
    Article Snippet: Samples were acquired using a flow cytometer (MACSQuant Analyzer 16, Miltenyi Biotec, USA) or sorted by fluorescence- activated cell sorting (FACS) using a BD Influx (BD Biosciences, USA).

    Article Title: Rifabutin boosts rifampicin accumulation in THP-1-derived M2 macrophages by inhibiting P-glycoprotein efflux activity.
    Article Snippet: Following this efflux phase, cells were washed two times and median fluorescence was recorded using a flow cytometer (MACSQuant analyzer 10, Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Clinical validation of CD16b as a standardized biomarker for inherited GPI deficiencies.
    Article Snippet: The stained cells were then analyzed by a flow cytometer (MACSQuant VYB, Miltenyi Biotec) to detect GPI-APs on granulocytes.

    Article Title: Influence of stabilizer, size and physical state of lipid nanoparticles on uptake by THP-1 macrophages.
    Article Snippet: The samples were protected from light and stored on ice until they were analyzed with a flow cytometer (MACS Quant VYB, Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Response of the bovine oviduct epithelium to sperm binding and proximity: a region-specific proteomic approach using spheroids.
    Article Snippet: Spermatozoa were then analyzed using a flow cytometer (Miltenyi Biotec MACSQuant Analyzer 10).

    Article Title: Development of highly selective, low-toxicity and orally available Mcl-1 inhibitors based on 3,5-dimethyl-4-sulfonimide-1H-pyrrole scaffold via P2/P3 dual pocket binding optimization.
    Article Snippet: Myeloid cell leukemia 1 (Mcl-1) is a pivotal anti-apoptotic protein, whose overexpression drives tumorigenesis, progression and drug resistance in multiple hematological malignancies.. Herein, guided by a bioisosteric design, we replaced the sulfonyl group with a drug-like sulfonimide linker and synthesized a novel series of Mcl-1 inhibitors based on our previous lead compound DDO-8201.. Among them, compound 52 displayed potent and selective Mcl-1 inhibition (Ki = 0.024 μM) with at least 400-fold selectivity over other Bcl-2 family proteins.

    Article Title: Metabolic rewiring driven by phosphoglycolate phosphatase deletion inhibits ferroptosis
    Article Snippet: Cell viability was assessed after 16 hours using resazurin (Cayman Chemical) or AquaBluer (MoBiTec), according to the manufacturer’s recommendations.

    Staining:

    Article Title: Impact of rifampicin on P-glycoprotein (ABCB1) expression in M1 and M2 macrophages derived from the THP-1 monocytic cell line or peripheral blood mononuclear cells
    Article Snippet: The obtained pellet was resuspended again in DPBS and the cell count of monocytes contained therein was determined using a flow cytometer (MACSQuant analyzer 10, Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Tunnelling Nanotube-Mediated Lysosome Sharing Promotes Osteocyte Survival via Transcellular Autophagy.
    Article Snippet: Samples were acquired using a flow cytometer (MACSQuant Analyzer 16, Miltenyi Biotec, USA) or sorted by fluorescence- activated cell sorting (FACS) using a BD Influx (BD Biosciences, USA).

    Article Title: Rifabutin boosts rifampicin accumulation in THP-1-derived M2 macrophages by inhibiting P-glycoprotein efflux activity.
    Article Snippet: Following this efflux phase, cells were washed two times and median fluorescence was recorded using a flow cytometer (MACSQuant analyzer 10, Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Clinical validation of CD16b as a standardized biomarker for inherited GPI deficiencies.
    Article Snippet: The stained cells were then analyzed by a flow cytometer (MACSQuant VYB, Miltenyi Biotec) to detect GPI-APs on granulocytes.

    Article Title: Influence of stabilizer, size and physical state of lipid nanoparticles on uptake by THP-1 macrophages.
    Article Snippet: The samples were protected from light and stored on ice until they were analyzed with a flow cytometer (MACS Quant VYB, Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Response of the bovine oviduct epithelium to sperm binding and proximity: a region-specific proteomic approach using spheroids.
    Article Snippet: Spermatozoa were then analyzed using a flow cytometer (Miltenyi Biotec MACSQuant Analyzer 10).

    Article Title: Development of highly selective, low-toxicity and orally available Mcl-1 inhibitors based on 3,5-dimethyl-4-sulfonimide-1H-pyrrole scaffold via P2/P3 dual pocket binding optimization.
    Article Snippet: Myeloid cell leukemia 1 (Mcl-1) is a pivotal anti-apoptotic protein, whose overexpression drives tumorigenesis, progression and drug resistance in multiple hematological malignancies.. Herein, guided by a bioisosteric design, we replaced the sulfonyl group with a drug-like sulfonimide linker and synthesized a novel series of Mcl-1 inhibitors based on our previous lead compound DDO-8201.. Among them, compound 52 displayed potent and selective Mcl-1 inhibition (Ki = 0.024 μM) with at least 400-fold selectivity over other Bcl-2 family proteins.

    Article Title: Metabolic rewiring driven by phosphoglycolate phosphatase deletion inhibits ferroptosis
    Article Snippet: Cell viability was assessed after 16 hours using resazurin (Cayman Chemical) or AquaBluer (MoBiTec), according to the manufacturer’s recommendations.

    Red Blood Cell Lysis:

    Article Title: Impact of rifampicin on P-glycoprotein (ABCB1) expression in M1 and M2 macrophages derived from the THP-1 monocytic cell line or peripheral blood mononuclear cells
    Article Snippet: The obtained pellet was resuspended again in DPBS and the cell count of monocytes contained therein was determined using a flow cytometer (MACSQuant analyzer 10, Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Tunnelling Nanotube-Mediated Lysosome Sharing Promotes Osteocyte Survival via Transcellular Autophagy.
    Article Snippet: Samples were acquired using a flow cytometer (MACSQuant Analyzer 16, Miltenyi Biotec, USA) or sorted by fluorescence- activated cell sorting (FACS) using a BD Influx (BD Biosciences, USA).

    Article Title: Rifabutin boosts rifampicin accumulation in THP-1-derived M2 macrophages by inhibiting P-glycoprotein efflux activity.
    Article Snippet: Following this efflux phase, cells were washed two times and median fluorescence was recorded using a flow cytometer (MACSQuant analyzer 10, Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Clinical validation of CD16b as a standardized biomarker for inherited GPI deficiencies.
    Article Snippet: The stained cells were then analyzed by a flow cytometer (MACSQuant VYB, Miltenyi Biotec) to detect GPI-APs on granulocytes.

    Article Title: Influence of stabilizer, size and physical state of lipid nanoparticles on uptake by THP-1 macrophages.
    Article Snippet: The samples were protected from light and stored on ice until they were analyzed with a flow cytometer (MACS Quant VYB, Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Response of the bovine oviduct epithelium to sperm binding and proximity: a region-specific proteomic approach using spheroids.
    Article Snippet: Spermatozoa were then analyzed using a flow cytometer (Miltenyi Biotec MACSQuant Analyzer 10).

    Article Title: Development of highly selective, low-toxicity and orally available Mcl-1 inhibitors based on 3,5-dimethyl-4-sulfonimide-1H-pyrrole scaffold via P2/P3 dual pocket binding optimization.
    Article Snippet: Myeloid cell leukemia 1 (Mcl-1) is a pivotal anti-apoptotic protein, whose overexpression drives tumorigenesis, progression and drug resistance in multiple hematological malignancies.. Herein, guided by a bioisosteric design, we replaced the sulfonyl group with a drug-like sulfonimide linker and synthesized a novel series of Mcl-1 inhibitors based on our previous lead compound DDO-8201.. Among them, compound 52 displayed potent and selective Mcl-1 inhibition (Ki = 0.024 μM) with at least 400-fold selectivity over other Bcl-2 family proteins.

    Article Title: Metabolic rewiring driven by phosphoglycolate phosphatase deletion inhibits ferroptosis
    Article Snippet: Cell viability was assessed after 16 hours using resazurin (Cayman Chemical) or AquaBluer (MoBiTec), according to the manufacturer’s recommendations.

    Control:

    Article Title: Impact of rifampicin on P-glycoprotein (ABCB1) expression in M1 and M2 macrophages derived from the THP-1 monocytic cell line or peripheral blood mononuclear cells
    Article Snippet: The obtained pellet was resuspended again in DPBS and the cell count of monocytes contained therein was determined using a flow cytometer (MACSQuant analyzer 10, Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Tunnelling Nanotube-Mediated Lysosome Sharing Promotes Osteocyte Survival via Transcellular Autophagy.
    Article Snippet: Samples were acquired using a flow cytometer (MACSQuant Analyzer 16, Miltenyi Biotec, USA) or sorted by fluorescence- activated cell sorting (FACS) using a BD Influx (BD Biosciences, USA).

    Article Title: Rifabutin boosts rifampicin accumulation in THP-1-derived M2 macrophages by inhibiting P-glycoprotein efflux activity.
    Article Snippet: Following this efflux phase, cells were washed two times and median fluorescence was recorded using a flow cytometer (MACSQuant analyzer 10, Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Clinical validation of CD16b as a standardized biomarker for inherited GPI deficiencies.
    Article Snippet: The stained cells were then analyzed by a flow cytometer (MACSQuant VYB, Miltenyi Biotec) to detect GPI-APs on granulocytes.

    Article Title: Influence of stabilizer, size and physical state of lipid nanoparticles on uptake by THP-1 macrophages.
    Article Snippet: The samples were protected from light and stored on ice until they were analyzed with a flow cytometer (MACS Quant VYB, Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Response of the bovine oviduct epithelium to sperm binding and proximity: a region-specific proteomic approach using spheroids.
    Article Snippet: Spermatozoa were then analyzed using a flow cytometer (Miltenyi Biotec MACSQuant Analyzer 10).

    Article Title: Development of highly selective, low-toxicity and orally available Mcl-1 inhibitors based on 3,5-dimethyl-4-sulfonimide-1H-pyrrole scaffold via P2/P3 dual pocket binding optimization.
    Article Snippet: Myeloid cell leukemia 1 (Mcl-1) is a pivotal anti-apoptotic protein, whose overexpression drives tumorigenesis, progression and drug resistance in multiple hematological malignancies.. Herein, guided by a bioisosteric design, we replaced the sulfonyl group with a drug-like sulfonimide linker and synthesized a novel series of Mcl-1 inhibitors based on our previous lead compound DDO-8201.. Among them, compound 52 displayed potent and selective Mcl-1 inhibition (Ki = 0.024 μM) with at least 400-fold selectivity over other Bcl-2 family proteins.

    Article Title: Metabolic rewiring driven by phosphoglycolate phosphatase deletion inhibits ferroptosis
    Article Snippet: Cell viability was assessed after 16 hours using resazurin (Cayman Chemical) or AquaBluer (MoBiTec), according to the manufacturer’s recommendations.

    Cell Characterization:

    Article Title: Impact of rifampicin on P-glycoprotein (ABCB1) expression in M1 and M2 macrophages derived from the THP-1 monocytic cell line or peripheral blood mononuclear cells
    Article Snippet: The obtained pellet was resuspended again in DPBS and the cell count of monocytes contained therein was determined using a flow cytometer (MACSQuant analyzer 10, Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Tunnelling Nanotube-Mediated Lysosome Sharing Promotes Osteocyte Survival via Transcellular Autophagy.
    Article Snippet: Samples were acquired using a flow cytometer (MACSQuant Analyzer 16, Miltenyi Biotec, USA) or sorted by fluorescence- activated cell sorting (FACS) using a BD Influx (BD Biosciences, USA).

    Article Title: Rifabutin boosts rifampicin accumulation in THP-1-derived M2 macrophages by inhibiting P-glycoprotein efflux activity.
    Article Snippet: Following this efflux phase, cells were washed two times and median fluorescence was recorded using a flow cytometer (MACSQuant analyzer 10, Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Clinical validation of CD16b as a standardized biomarker for inherited GPI deficiencies.
    Article Snippet: The stained cells were then analyzed by a flow cytometer (MACSQuant VYB, Miltenyi Biotec) to detect GPI-APs on granulocytes.

    Article Title: Influence of stabilizer, size and physical state of lipid nanoparticles on uptake by THP-1 macrophages.
    Article Snippet: The samples were protected from light and stored on ice until they were analyzed with a flow cytometer (MACS Quant VYB, Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Response of the bovine oviduct epithelium to sperm binding and proximity: a region-specific proteomic approach using spheroids.
    Article Snippet: Spermatozoa were then analyzed using a flow cytometer (Miltenyi Biotec MACSQuant Analyzer 10).

    Article Title: Development of highly selective, low-toxicity and orally available Mcl-1 inhibitors based on 3,5-dimethyl-4-sulfonimide-1H-pyrrole scaffold via P2/P3 dual pocket binding optimization.
    Article Snippet: Myeloid cell leukemia 1 (Mcl-1) is a pivotal anti-apoptotic protein, whose overexpression drives tumorigenesis, progression and drug resistance in multiple hematological malignancies.. Herein, guided by a bioisosteric design, we replaced the sulfonyl group with a drug-like sulfonimide linker and synthesized a novel series of Mcl-1 inhibitors based on our previous lead compound DDO-8201.. Among them, compound 52 displayed potent and selective Mcl-1 inhibition (Ki = 0.024 μM) with at least 400-fold selectivity over other Bcl-2 family proteins.

    Article Title: Metabolic rewiring driven by phosphoglycolate phosphatase deletion inhibits ferroptosis
    Article Snippet: Cell viability was assessed after 16 hours using resazurin (Cayman Chemical) or AquaBluer (MoBiTec), according to the manufacturer’s recommendations.



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